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A few plasmids (pGL1124, pGL1224 and pGL1217) ended up created to enable the alternative of one allele of CYC9To analyze the subcellular localization from the CRK12 protein, a pEarleyGate104 vector was employed for a transient expression of the CRK12 protein fused to yellow fluorescent protein (YFP). The confocal illustrations or photos from the P.